From:PHS POLICY ON HUMANE CARE AND USE OF LABORATORY ANIMALS CLARIFICATION REGARDING USE OF CARBON DIOXIDE FOR EUTHANASIA OF SMALL LABORATORY ANIMALS; Release Date: July 17, 2002; NOTICE: NOT-OD-02-062
“REPORTING OF NONCOMPLIANCE
Unintended recovery of animals after apparent death from CO2 (e.g., in necropsy coolers) is a documented occurrence. Institutions are reminded that such incidents constitute serious noncompliance with the PHS Policy and serious deviation from the provisions of the Guide for the Care and Use of Laboratory Animals (http://www.nap.edu/readingroom/books/labrats/). As such, the IACUC, through the Institutional Official, must promptly provide OLAW with a full explanation of the circumstances and actions taken. Prompt reporting in accordance with PHS Policy requirements (PHS Policy IV.F.3) is an essential component of the formal relationship between OLAW and PHS-Assured institutions.”
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